Charlie Kirk’s assassin remains at large after a person of interest previously was released from custody — marking the second individual to be detained and let go by police scrambling to find the conservative icon’s killer.

“The subject in custody has been released after an interrogation by law enforcement,” FBI Director Kash Patel shockingly announced Wednesday evening.

“Our investigation continues and we will continue to release information in interest of transparency,” Patel added.

Law enforcement’s stunning reversal comes not even two hours after Patel proclaimed in a tweet that Kirk’s killer had been captured – with law enforcement and Utah Gov. Spencer Cox holding a press conference moments later announcing security footage had led them to a person of interest.

The Post spoke to the mother of the now-exonerated person of interest, who said she was “super shaken” by the gruesome shooting he witnessed firsthand – and then by his subsequent detention, his frightened mother told The Post.

“He’s a great kid,” the mother said.

“I have a picture of him. He’s like 25-feet from Charlie on the other side that he was shot. He sent us a video.”

And the man was a Charlie Kirk fan, and had travelled to the Utah Valley Campus Wednesday – only to watch him violently murdered, and then be blamed for the it.

“He was attending like any other college kid. He listens to him,” the mother said.

That person of interest was just the second detained and then released since the 31-year-old was gunned down on the Utah Valley University campus just after noon local time Wednesday.

The first — an older man seen with his pants around his ankles — was seen being dragged away by cops who told the screaming crowds that the man had confessed.

That individual was also later cleared – and means the person who murdered one of America’s most prominent conservative voices is in the wind.